thp 1 dualtm sting reporter cells (InvivoGen)
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thp 1 dualtm sting reporter cells
Thp 1 Dualtm Sting Reporter Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thp+1+dualtm+sting+reporter+cells/THP1-Dual+Cells/us12673954-2746-0-5
Average 98 stars, based on 424 article reviews
Thp 1 Dualtm Sting Reporter Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 98/100, based on 424 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/thp+1+dualtm+sting+reporter+cells/THP1-Dual+Cells/us12673954-2746-0-5
Average 98 stars, based on 424 article reviews
thp 1 dualtm sting reporter cells - by Bioz Stars,
2026-10
98/100 stars
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Clinical Proteomics:Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: THP-1 DualTM STING reporter cells (Invivogen, Dual cells cat #THPD-nfis) were added to the plates with compounds at 15,000 cells in 10 μL per well in RPMI media (Gibco, cat #11875) containing 10% human plasma in a low volume 384-well black wall clear bottom tissue culture plate (Corning, cat #3542) for SEAP assay and low volume solid white plate (Corning, cat #3826) for luciferase assay. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: The crude material was purified via preparative HPLC with the following conditions: Column: Agilent Bonus RP 21.2×100 mm, 5-μm particles; Mobile Phase A: water with 20-mM ammonium acetate: Mobile Phase B: acetonitrile; Gradient: 0% B hold 0-6 minute. .. Article Title: Benzofuran compounds as sting agonists Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: HPLC: RT=0.38 min (Waters Acquity UPLC BEH C18, 2.1×50 mm, 1.7-μm particles; Mobile Phase A: water with 0.05% TFA; Mobile Phase B: acetonitrile with 0.05% TFA; Temperature: 50° C.; Gradient: 2-98% B over 1 minute, then 98-2% B over 0.5 min; Flow: 0.8 mL/min.); MS (ES): m/z=661.2 [M+H]+. .. SEAP Assay:Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: THP-1 DualTM STING reporter cells (Invivogen, Dual cells cat #THPD-nfis) were added to the plates with compounds at 15,000 cells in 10 μL per well in RPMI media (Gibco, cat #11875) containing 10% human plasma in a low volume 384-well black wall clear bottom tissue culture plate (Corning, cat #3542) for SEAP assay and low volume solid white plate (Corning, cat #3826) for luciferase assay. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: The crude material was purified via preparative HPLC with the following conditions: Column: Agilent Bonus RP 21.2×100 mm, 5-μm particles; Mobile Phase A: water with 20-mM ammonium acetate: Mobile Phase B: acetonitrile; Gradient: 0% B hold 0-6 minute. .. Article Title: Benzofuran compounds as sting agonists Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: HPLC: RT=0.38 min (Waters Acquity UPLC BEH C18, 2.1×50 mm, 1.7-μm particles; Mobile Phase A: water with 0.05% TFA; Mobile Phase B: acetonitrile with 0.05% TFA; Temperature: 50° C.; Gradient: 2-98% B over 1 minute, then 98-2% B over 0.5 min; Flow: 0.8 mL/min.); MS (ES): m/z=661.2 [M+H]+. .. Luciferase:Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: THP-1 DualTM STING reporter cells (Invivogen, Dual cells cat #THPD-nfis) were added to the plates with compounds at 15,000 cells in 10 μL per well in RPMI media (Gibco, cat #11875) containing 10% human plasma in a low volume 384-well black wall clear bottom tissue culture plate (Corning, cat #3542) for SEAP assay and low volume solid white plate (Corning, cat #3826) for luciferase assay. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: The crude material was purified via preparative HPLC with the following conditions: Column: Agilent Bonus RP 21.2×100 mm, 5-μm particles; Mobile Phase A: water with 20-mM ammonium acetate: Mobile Phase B: acetonitrile; Gradient: 0% B hold 0-6 minute. .. Article Title: Benzofuran compounds as sting agonists Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: Serial dilutions of compounds in DMSO were added to low volume 384 well plates at 100 nl/well using an ECHO acoustic dispenser (Labcyte, model 550) to achieve final starting concentration of 100 μM in cell suspension. .. Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: HPLC: RT=0.38 min (Waters Acquity UPLC BEH C18, 2.1×50 mm, 1.7-μm particles; Mobile Phase A: water with 0.05% TFA; Mobile Phase B: acetonitrile with 0.05% TFA; Temperature: 50° C.; Gradient: 2-98% B over 1 minute, then 98-2% B over 0.5 min; Flow: 0.8 mL/min.); MS (ES): m/z=661.2 [M+H]+. .. other:Article Title: Cyclic dinucleotides as anticancer agents Article Snippet: The crude product was dissolved in a small amount of MeOH, adsorbed onto a plug of Celite and purified on a reverse phase ISCO Gold 50 g C18 column (Mobile Phase A: 5:95 acetonitrile:water with 0.01M ammonium acetate; Mobile Phase B: 95:5 acetonitrile:water with 0.01M ammonium acetate; Gradient: 0% B hold for 5 min, 0-40% B gradient for 20 min and 100% hold for 5 min, run at 30 mL/min) to afford Intermediate 22B (254.7 mg, 68.1% yield) as a white solid after lyophilization. |